Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift | Zendy

Vaculovicova Marketa | Zendy; Adam Vojtech | Zendy; Kizek Rene | Zendy

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Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift

Author(s) -

Vaculovicova Marketa,

Adam Vojtech,

Kizek Rene

Publication year - 2015

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.201500168

Subject(s) - electrophoretic mobility shift assay , bioconjugation , electrophoresis , biomolecule , nanomaterials , chemistry , dna , nanotechnology , chromatography , materials science , combinatorial chemistry , biochemistry , gene , gene expression

Electrophoretic mobility shift assay (EMSA) is a well‐established technique to monitor interactions between biomolecules particularly DNA and proteins. Even though numerous variations of this method have been presented, challenges in the form of detection sensitivity and/or variations in the stability of the formed complex still remain. With advances in the area of nanomaterials improvements in EMSA have been also suggested. Recently, Zhang and Wang ( Electrophoresis 2015, 36 , 1011–1015) presented electrophoretic mobility shift method for determination of number of DNA molecules conjugated to quantum dots (QDs), which was further utilized for calculation of enzymatic activity, sequence specific DNA detection, and neutral molecule quantification.

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