Premium
Capillary electrophoretic study of green fluorescent hollow carbon nanoparticles
Author(s) -
Liu Lizhen,
Feng Feng,
Hu Qin,
Paau Man Chin,
Liu Yang,
Chen Zezhong,
Bai Yunfeng,
Guo Fangfang,
Choi Martin M. F.
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500166
Subject(s) - fluorescence , photoluminescence , capillary electrophoresis , electrophoresis , absorption (acoustics) , quantum yield , nanoparticle , buffer (optical fiber) , analytical chemistry (journal) , materials science , absorption spectroscopy , spectroscopy , transmission electron microscopy , phosphate buffered saline , phosphate , colloid , chemistry , nanotechnology , chromatography , optoelectronics , organic chemistry , optics , telecommunications , physics , quantum mechanics , computer science , composite material
CE coupled with laser‐induced fluorescence and UV absorption detections has been applied to study the complexity of as‐synthesized green fluorescent hollow carbon nanoparticles (HC‐NP) samples. The effects of pH, type, and concentration of the run buffer and SDS on the separation of HC‐NP are studied in detail. It is observed that phosphate run buffer is more effective in separating the HC‐NP and the optimal run buffer is found to be 30 mM phosphate and 10 mM SDS at pH 9.0. The CE separation of this HC‐NP is based on the difference in size and electrophoretic mobility of HC‐NP. Some selected HC‐NP fractions are collected and further characterized by UV‐visible absorption and photoluminescence (PL) spectroscopy, MS, and transmission electron microscopy. The fractionated HC‐NP show profound differences in absorption, emission characteristics, and PL quantum yield that would have been otherwise misled by studying the complex mixture alone. It is anticipated that our CE methodology will open a new initiative on extensive studies of individual HC‐NP species in the biomedical, catalysis, electronic, and optical device, energy storage, material, and sensing field.