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Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection
Author(s) -
Thang Lee Yien,
Shahir Shafinaz,
See Hong Heng
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500164
Subject(s) - chromatography , chemistry , analyte , tamoxifen , detection limit , capillary electrophoresis , acetic acid , breast cancer , medicine , cancer , biochemistry
A new approach for the quantification of tamoxifen and its metabolites 4‐hydroxytamoxifen, N ‐desmethyltamoxifen, and 4‐hydroxy‐ N ‐desmethyltamoxifen (endoxifen) in human plasma samples using NACE coupled with contactless conductivity detection (C 4 D) is presented. The buffer system employed consisted of 7.5 mM deoxycholic acid sodium salt, 15 mM acetic acid, and 1 mM 18‐crown‐6 in 100% methanol. The complete separation of all targeted compounds (including endoxifen racemate) could be achieved within 6 min under optimized conditions. The proposed method was validated and showed good linearity in the range from 100 to 5000 ng/mL with correlation coefficients between 0.9922 and 0.9973, LODs in the range of 25–40 ng/mL, and acceptable reproducibility of the peak area (intraday RSD 2.2–3.1%, n = 4; interday (3 days) RSD 6.0–8.8%, n = 4). The developed method was successfully demonstrated for the quantification of tamoxifen and its metabolites in human plasma samples collected from breast cancer patients undertaking tamoxifen treatment.