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Aptamer‐based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection
Author(s) -
Liu YanMing,
Cao JunTao,
Liu YingYing,
Zhang JingJing,
Zhou Min,
Huang KeJing,
Chen YongHong,
Ren ShuWei
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500158
Subject(s) - aptamer , chemiluminescence , detection limit , luminol , chemistry , capillary electrophoresis , immunoglobulin e , chromatography , colloidal gold , linear range , antibody , microbiology and biotechnology , nanoparticle , nanotechnology , biology , materials science , immunology
A novel aptamer‐based CE with chemiluminescence (CL) assay was developed for highly sensitive detection of human immunoglobulin E (IgE). The IgE aptamer was conjugated with gold nanoparticles (AuNPs) to form AuNPs‐aptamer that could specifically recognize the IgE to produce an AuNPs‐aptamer‐IgE complex. The mixture of the AuNPs‐aptamer‐IgE complex and the unbounded AuNPs‐aptamer could be effectively separated by CE and sensitively detected with luminol‐H 2 O 2 CL system. By taking the advantage of the excellent catalytic behavior of AuNPs on luminol‐H 2 O 2 CL system, the ultrasensitive detection of IgE was achieved. The detection limit of IgE is 7.6 fM ( S/N = 3) with a linear range from 0.025 to 250 pM. Successful detection of IgE in human serum samples was demonstrated and the recoveries of 94.9–103.2% were obtained. The excellent assay features of the developed approach are its specificity, sensitivity, adaptability, and very small sample consumption. Our design provides a methodology model for determination of rare proteins in biological samples.