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Solid phase extraction methodology for UPLC‐MS based metabolic profiling of urine samples
Author(s) -
Michopoulos Filippos,
Gika Helen,
Palachanis Dimitrios,
Theodoridis Georgios,
Wilson Ian D.
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500101
Subject(s) - chromatography , chemistry , solid phase extraction , fractionation , sample preparation , urine , hydrophilic interaction chromatography , extraction (chemistry) , alkyl , metabolomics , high performance liquid chromatography , organic chemistry , biochemistry
In general, when performing untargeted metabolic phenotyping (metabolomics/metabonomics) studies on biological samples for example urine or food, sample preparation should be kept to a minimum. However, there are circumstances when desalting, preconcentration, or the fractionation of samples into polar and nonpolar metabolites is of value for enabling the subsequent analysis. Because of its simplicity and ease of automation SPE is well suited to such applications prior to analysis by ultra‐performance LC‐TOF‐MS. In the present study, the properties of a range of SPE phases have been investigated with respect to the range of metabolites that can be extracted from urine. The phases include alkyl modified (C8 and, C18‐OH and C18) silica and polymeric materials. The results show that the C18 phase was well suited to fractionating urine into samples suitable for separate analysis of polar and nonpolar constituents via HILIC and RPLC, respectively, while the polymeric materials were best for concentrating and desalting samples.