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Microchip with an open tubular immobilized ph gradient for UV whole column imaging detection
Author(s) -
Zeng Hulie,
Glawdel Tomasz,
Ren Carolyn L.
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500041
Subject(s) - chromatography , analytical chemistry (journal) , channel (broadcasting) , column (typography) , immobilized ph gradient , dynamic range , chemistry , materials science , computer science , isoelectric focusing , computer network , telecommunications , biochemistry , frame (networking) , enzyme , computer vision
This study reports a new method for establishing an open tubular IPG in a microchip coupled with a whole column image detection (WCID) system for protein separation applications. This method allows a wider range of immobilized pH (2.6–9.5) to be established in a PDMS/quartz channel by controlling the diffusion of acidic and basic polymer solutions into the channel through well‐designed channel dimensions. The developed pH gradient was experimentally validated by performing the separation of a mixture of standard p I markers. It was further validated by the separation of the hemoglobin control AFSC sample. This method is advantageous over existing IPG methods because it has a wider range of pH and maintains the open tubular feature that matches the UV WCID to improve the sensitivity.