Premium
Analysis of small interfering RNA by capillary electrophoresis in hydroxyethylcellulose solutions
Author(s) -
Liu Chenchen,
Yamaguchi Yoshinori,
Zhu Xifang,
Li Zhenqing,
Ni Yi,
Dou Xiaoming
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500018
Subject(s) - rna silencing , rna , small interfering rna , microbiology and biotechnology , capillary electrophoresis , electrophoresis , chemistry , chromatography , polymer , biophysics , biology , rna interference , gene , biochemistry , organic chemistry
The analysis of small interfering RNA (siRNA) is important for gene function studies and drug developments. We employed CE to study the separation of siRNA ladder marker, which were ten double‐stranded RNA (dsRNA) fragments ranged from 20 to 1000 bp, in solutions of hydroxyethylcellulose (HEC) polymer with different concentrations and molecular weights ( Mw s). Migration mechanism of dsRNA during CE was studied by the mobility and resolution length ( RL ) plots. We found that the RL depended on not only the concentration of HEC, but also the Mw of HEC. For instance, RL of small dsRNA fragment was more influenced by concentration of high Mw HEC than large dsRNA fragment and RL of large dsRNA fragment was more influenced by concentration of low Mw HEC than small dsRNA fragment. In addition, we found electrophoretic evidence that the structure of dsRNA was more compact than dsDNA with the same length. In practice, we succeeded to separate the glyceraldehyde 3‐phosphate dehydrogenase siRNA in the mixture of the siRNA ladder marker within 4 min.