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One‐day pulsed‐field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates
Author(s) -
Kaminska Paulina S.,
Fiedoruk Krzysztof,
Jankowska Dominika,
Mahillon Jacques,
Nowosad Karol,
Drewicka Ewa,
Zambrzycka Monika,
Swiecicka Izabela
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400552
Subject(s) - bacillus cereus , cereus , bacilli , microbiology and biotechnology , biology , spore , pulsed field gel electrophoresis , food poisoning , bacteria , gel electrophoresis , toxin , bacillus (shape) , genotype , gene , biochemistry , genetics
Bacillus cereus , the Gram‐positive and spore‐forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat‐stable emetic toxin. Rapid determination of cereulide‐positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1‐day pulsed‐field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.