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Searching for biomarkers of comorbidities in sera of treated HIV‐infected patients by isoelectric focusing
Author(s) -
Malvoisin Etienne,
Makhloufi Djamila,
Livrozet JeanMichel
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400535
Subject(s) - gastroenterology , medicine , isoelectric focusing , alkaline phosphatase , glutathione , human immunodeficiency virus (hiv) , glutathione s transferase , immunology , enzyme , biology , biochemistry
Based on their characteristics, we hypothesized that the following parameters, namely collagen IV, glutathione S‐transferase, secretory component (SC), and AMP‐activated protein kinase α1α2 may be useful serum markers in the detection of comorbidities in treated HIV‐infected patients. These parameters were determined in 204 HIV‐infected patients and 35 controls by using IEF and densitometry. Collagen IV was undetectable in controls and the majority of HIV‐infected patients. Twenty‐two HIV‐infected patients presented significantly elevated levels of collagen IV, most of them were coinfected with hepatitis C virus and/or hepatitis B virus. SC was undetectable in controls. SC was significantly increased in 81 HIV‐infected patients and significantly correlated with aspartate aminotransferase ( r = 0.267, p = 0.0049), alkaline phosphatase ( r = 0.309, p = 0.0011), and γ‐glutamyl‐transferase ( r = 0.264, p = 0.0054). Glutathione S ‐transferase levels of HIV‐infected patients were significantly higher than the controls (3779 ± 5860 vs. 785 ± 71 DU, p = 0.0007) and significantly correlated with serum urea ( r = 0.204, p = 0.0038), triglycerides ( r = 0.209, p = 0.0033), and lipase ( r = 0.219, p = 0.0025). AMP‐activated protein kinase α1α2 levels of HIV‐infected patients were significantly higher than the controls (5676 ± 6248 vs. 1189 ± 6248 DU, p = 0.0009). Further studies are needed to demonstrate the relevance of these results to diagnose non‐AIDS‐related illnesses in HIV‐infected patients.