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Expanding the scope of pressure‐assisted electrokinetic injection for online concentration of positively charged analytes in capillary electrophoresis‐mass spectrometry
Author(s) -
D'Ulivo Lucia,
Feng YongLai
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400514
Subject(s) - electrokinetic phenomena , analyte , repeatability , capillary electrophoresis , chromatography , detection limit , chemistry , mass spectrometry , analytical chemistry (journal) , calibration , spark plug , statistics , mathematics , engineering , aerospace engineering
Sample injection is a crucial step in CE. In past, many efforts have been focused on concentrating the analytes into a sharp sample plug. In 2006, pressure‐assisted electrokinetic injection (PAEKI) was proposed as a new preconcentration technique for anions. In this study, we expanded the applicability of PAEKI to online preconcentrate positively charged analytes. l ‐Arginine, l ‐lysine, and imidazole were chosen as target analytes for method development. The enhancement factor of PAEKI was over 3000‐fold. When CZE was coupled with a Q‐TOF system, PAEKI delivers a detection limit of 18–28 pg/mL and a dynamic calibration range over four orders of magnitude. The RSD was less than 6.4% ( n = 4) on both peak area and migration time, indicating a good repeatability.