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Dielectrophoresis chips improve PCR detection of the food‐spoiling yeast Zygosaccharomyces rouxii in apple juice
Author(s) -
del Carmen Jaramillo Maria,
Huttener Mario,
Alvarez Juan Manuel,
HomsCorbera Antoni,
Samitier Josep,
Torrents Eduard,
Juárez Antonio
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400483
Subject(s) - yeast , dielectrophoresis , population , food science , chromatography , chemistry , biology , biochemistry , electrode , demography , sociology
Dielectrophoretic (DEP) manipulation of cells present in real samples is challenging. We show in this work that an interdigitated DEP chip can be used to trap and wash a population of the food‐spoiling yeast Zygosaccharomyces rouxii that contaminates a sample of apple juice. By previously calibrating the chip, the yeast population loaded is efficiently trapped, washed, and recovered in a small‐volume fraction that, in turn, can be used for efficient PCR detection of this yeast. DEP washing of yeast cells gets rid of PCR inhibitors present in apple juice and facilitates PCR analysis. This and previous works on the use of DEP chips to improve PCR analysis show that a potential use of DEP is to be used as a treatment of real samples prior to PCR.