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Determination of beta‐agonists in swine hair by μFIA and chemiluminescence
Author(s) -
Chen Xu,
Luo Yong,
Shi Bo,
Gao Zhigang,
Du Yuguang,
Liu Xianming,
Zhao Weijie,
Lin Bingcheng
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400412
Subject(s) - ractopamine , terbutaline , chemiluminescence , luminol , potassium ferricyanide , salbutamol , chemistry , chromatography , microfluidic chip , microfluidics , materials science , nanotechnology , medicine , inorganic chemistry , asthma
β‐Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the β‐agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the β‐agonists. The chip was fabricated from two glass plates (64 mm × 32 mm) with microchannels of 200 μm width and 100 μm depth. The detection limits were achieved at 2.0 × 10 −8 mol/L of ractopamine, 1.0 × 10 −8 mol/L of terbutaline and 5.0 × 10 −7 mol/L of salbutamol. In this report, our method was applied for determination of the β‐agonists in swine hair from three different sources with satisfactory results.