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Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells
Author(s) -
Zhu Xiuzhen,
Chen Yi
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400344
Subject(s) - electrophoresis , dielectrophoresis , electric field , analytical chemistry (journal) , channel (broadcasting) , materials science , capillary electrophoresis , chemistry , biological system , chromatography , computer science , physics , telecommunications , biology , quantum mechanics
Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video for real‐time observation. The experiment can be performed within 0.044–1 s. In combination with digital calculation based on electromagnetic theory, the method was validated to be applicable to the determination of electrophoretic and dielectrophoretic mobilities, μ EP and μ DEP , of human blood erythrocytes, giving μ EP = −(0.87 ± 0.16)× 10 −4 cm 2 ·V −1 · s −1 and μ DEP = −(4.5 ± 1.3) × 10 −8 cm 4 ·V −2 ·s −1 by vector decomposition, or μ EP = −(0.89 ± 0.14) × 10 −4 cm 2 ·V −1 · s −1 and μ DEP = −(4.6 ±1.2) × 10 −8 cm 4 ·V −2 · s −1 by least squares fitting, all agreeing with published data. Hydrodynamic and EOFs were eliminated for better measurement. It was found that the location of cells had a serious impact on the measurement precision, and the upstream of the cross channel along the electric field was chosen for precise measurement. The method is also extendable to the study of other cells and particles.