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Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application
Author(s) -
Zhu BoFeng,
Zhang YuDang,
Shen ChunMei,
Du WeiAn,
Liu WenJuan,
Meng HaoTian,
Wang HongDan,
Yang Guang,
Jin Rui,
Yang ChunHua,
Yan JiangWei,
Bie XiaoHua
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400333
Subject(s) - amelogenin , multiplex , str analysis , forensic science , multiplex polymerase chain reaction , str multiplex system , dna , computational biology , dna profiling , biology , chromatography , microbiology and biotechnology , polymerase chain reaction , genetics , microsatellite , chemistry , gene , allele
In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex‐determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.