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Surface modification of PDMS microfluidic devices by controlled sulfuric acid treatment and the application in chip electrophoresis
Author(s) -
Gitlin Leonid,
Schulze Philipp,
Ohla Stefan,
Bongard HansJosef,
Belder Detlev
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400269
Subject(s) - microfluidics , surface modification , rhodamine b , electrophoresis , materials science , rhodamine , sulfuric acid , chemical engineering , fluorescence , x ray photoelectron spectroscopy , chemical modification , fourier transform infrared spectroscopy , chromatography , nanotechnology , analytical chemistry (journal) , chemistry , polymer chemistry , organic chemistry , physics , photocatalysis , quantum mechanics , engineering , metallurgy , catalysis
Herein, we present a straightforward surface modification technique for PDMS‐based microfluidic devices. The method takes advantage of the high reactivity of concentrated sulfuric acid to enhance the surface properties of PDMS bulk material. This results in alteration of the surface morphology and chemical composition that is in‐depth characterized by ATR‐FTIR, EDX, SEM, and XPS. In comparison to untreated PDMS, modified substrates exhibit a significantly reduced diffusive uptake of small organic molecules while retaining its low electroosmotic properties. This was demonstrated by exposing the channels of a microfluidic device to concentrated rhodamine B solution followed by fluorescence microscopy. The surface modification procedure was used to improve chip‐based electrophoretic separations. Separation efficiencies of FITC‐labeled amines/amino acids obtained in treated and untreated PDMS‐devices as well as in glass chips were compared. We obtained higher efficiencies in H 2 SO 4 treated PDMS chips compared to untreated ones but lower efficiencies than those obtained in commercial microfluidic glass devices.