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Dual characterization of biological cells by optofluidic microscope and resistive pulse sensor
Author(s) -
Guo Jinhong,
Chen Liang,
Huang Xiwei,
Li Chang Ming,
Ai Ye,
Kang Yuejun
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400268
Subject(s) - microfluidics , resistive touchscreen , software portability , characterization (materials science) , nanotechnology , materials science , microscope , microscopy , lab on a chip , microfluidic chip , computer science , optoelectronics , optics , physics , computer vision , programming language
Label‐free detection technique has emerged as a powerful platform for biomedical applications since it can avoid laborious multi‐step sample preparation. In this paper, we demonstrate a dual analysis of biological cells using a single microfluidic system combining optofluidic microscopy and resistive pulse sensing. Both red blood cells ( RBC s) and circulating tumor cells ( CTC s) have been used to validate the concept of dual analysis and also to test the performance of the microfluidic device. The cell characterization by resistive pulse sensing is in good agreement with the analysis by optofluidic microscopy, further verified by the commercial Beckman‐Coulter® FC 500 flow cytometry. The present system has attractive merits such as simple fabrication, easy integration, high portability, and low cost. This study has great potentials for the development of innovative on‐chip flow cytometry with concurrent imaging sensing and resistive sensing.