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Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics
Author(s) -
Fahrenkopf Max A.,
Mukherjee Tamal,
Ydstie B. Erik,
Schneider James W.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400266
Subject(s) - microfluidics , capillary electrophoresis , electrophoresis , chromatography , dna , dna sequencing , chemistry , nanotechnology , materials science , biochemistry
We present a nonlinear optimization study of different implementations of the DNA electrophoretic method “End‐labeled Free‐solution Electrophoresis” in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro‐osmotic counterflows and snap‐shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.