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Counting of Escherichia coli by a microflow cytometer based on a photonic–microfluidic integrated device
Author(s) -
Guo Tianyi,
Wei Yin,
Xu Changqing,
Watts Benjamin R.,
Zhang Zhiyi,
Fang Qiyin,
Zhang Haiying,
Selvaganapathy P. Ravi,
Deen M. Jamal
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400211
Subject(s) - hemocytometer , microfluidics , escherichia coli , materials science , photonics , chromatography , optoelectronics , analytical chemistry (journal) , nanotechnology , chemistry , biochemistry , gene
Counting of Escherichia coli DH5α‐cell suspensions in PBS is performed using a microflow cytometer based on a photonic–microfluidic integrated device. Side‐scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2–4 μm) as their scattering intensities are different.