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Multicapillary SDS‐gel electrophoresis for the analysis of fluorescently labeled mAb preparations: A high throughput quality control process for the production of QuantiPlasma and PlasmaScan mAb libraries
Author(s) -
Székely Andrea,
Szekrényes Ákos,
Kerékgyártó Márta,
Balogh Attila,
Kádas János,
Lázár József,
Guttman András,
Kurucz István,
Takács László
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400208
Subject(s) - monoclonal antibody , gel electrophoresis , proteome , chemistry , electrophoresis , chromatography , computational biology , microbiology and biotechnology , biochemistry , biology , antibody , immunology
Molecular heterogeneity of mAb preparations is the result of various co‐ and post‐translational modifications and to contaminants related to the production process. Changes in molecular composition results in alterations of functional performance, therefore quality control and validation of therapeutic or diagnostic protein products is essential. A special case is the consistent production of mAb libraries (QuantiPlasma™ and PlasmaScan™) for proteome profiling, quality control of which represents a challenge because of high number of mAbs (>1000). Here, we devise a generally applicable multicapillary SDS‐gel electrophoresis process for the analysis of fluorescently labeled mAb preparations for the high throughput quality control of mAbs of the QuantiPlasma™ and PlasmaScan™ libraries.