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Detection of S ‐adenosylhomocysteine and methylation index in blood by capillary electrophoresis
Author(s) -
Ivanov Alexander V.,
Luzyanin Boris P.,
Virus Edward D.,
Rotkina Anna S.,
Kubatiev Aslan A.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400160
Subject(s) - capillary electrophoresis , chromatography , detection limit , chemistry , reproducibility , dilution , capillary action , methylation , glycine , analytical chemistry (journal) , dna , biochemistry , materials science , amino acid , physics , composite material , thermodynamics
This work proposes an approach to the direct analysis of S ‐adenosylhomocysteine (SAH) and the methylation index in blood using CE with UV detection (CE‐UV). After application of meglumine postinjection, we achieved SAH in‐capillary preconcentration in the HClO 4 extracts of erythrocytes, which improved the detection limit ( S/N = 3) of SAH up to 3 fmol or 180 nmol/L at the injection volume of 50 nL, taking into account the sample dilution rate. CE‐UV was carried out in 30 mM glycine and 45 mmol/L HCl (pH ∼1.8) at 17 kV in a capillary 48 cm in length and 50 μm id. Accuracy of the technique was 101% and reproducibility was about 12%.
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