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Determination of muscimol and ibotenic acid in mushrooms of Amanitaceae by capillary electrophoresis
Author(s) -
Poliwoda Anna,
Zielińska Katarzyna,
Halama Marek,
Wieczorek Piotr P.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400104
Subject(s) - ibotenic acid , chemistry , chromatography , muscimol , capillary electrophoresis , sodium dodecyl sulfate , analyte , extraction (chemistry) , calibration curve , quantitative analysis (chemistry) , sodium , methanol , detection limit , biochemistry , gabaa receptor , organic chemistry , receptor , neuroscience , biology , central nervous system
In this study, the CZE method for rapid quantitative and qualitative determination of ibotenic acid and muscimol in Amanita mushrooms naturally grown in Poland was developed. The investigations included the species of A. muscaria , A. pantherina , and A. citrina , collected in southern region of Poland. The studied hallucinogenic compounds were effectively extracted with a mixture of methanol and 1 mM sodium phosphate buffer at pH 3 (1:1 v/v) using ultrasound‐assisted procedure. The obtained extracts were separated and determined by CZE utilizing a 25 mM sodium phosphate running buffer adjusted to pH 3 with 5% content of acetonitrile v/v. The calibration curves for both analytes were linear in the range of 2.5–7000 μg/mL. The intraday and interday variations of quantitative data were 1.0 and 2.5% RSD, respectively. The recovery values of analyzed compounds were over 87%. The identities of ibotenic acid and muscimol were confirmed by UV spectra, migration time, and measurements after addition of external standard.