Premium
Combining bar adsorptive microextraction with capillary electrophoresis—Application for the determination of phenolic acids in food matrices
Author(s) -
da Rosa Neng Nuno,
Sequeiros Rute C. P.,
Florêncio Nogueira José Manuel
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400101
Subject(s) - chromatography , chemistry , capillary electrophoresis , detection limit , aqueous solution , organic chemistry
In this contribution, bar adsorptive microextraction coated with a mixed‐mode anion exchange/RP followed by liquid desorption was combined for the first time with a capillary electrophoresis‐diode array detection system (BAμE(MAX)‐LD/CE‐DAD), for the determination of phenolic acids in food matrices, using chlorogenic, ferulic, cumaric, and caffeic acids as model compounds. Assays performed in aqueous media spiked at the 0.8 mg/L level yielded average recoveries up to 40% for all four phenolic acids, under optimized experimental conditions. The analytical performance showed also good precision (RSD < 15%), convenient LODs (18.0–85.0 μg/L) and linear dynamic ranges (0.8–8.0 mg/L) with convenient determination coefficients ( r 2 > 0.9900). By using the standard addition method, the application to food matrices such as green tea, red fruit juice, and honey allowed very good performances for the determination of minor amounts of phenolic acids. The proposed methodology proved to be a suitable alternative for the analysis of polar to ionic compounds, showing to be easy to implement, reliable, sensitive, and requiring a low sample volume to determine phenolic acids in food samples.