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Interface solution isoelectric focusing with in situ MALDI ‐ TOF mass spectrometry
Author(s) -
Wang Shujun,
Chen Suming,
Wang Jianing,
Xu Peng,
Luo Yuanming,
Nie Zongxiu,
Du Wenbin
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400083
Subject(s) - isoelectric focusing , chromatography , microfluidics , mass spectrometry , chemistry , in situ , electrophoresis , resolution (logic) , analytical chemistry (journal) , materials science , nanotechnology , computer science , biochemistry , organic chemistry , artificial intelligence , enzyme
This paper describes a simple and reusable microfluidic device combining solution IEF (s IEF ) with MALDI‐TOF MS for rapid proteomic and metabolic analysis of microliter samples. The device contains two glass plates with nanoliter microwell arrays, which can be assembled to form a fluidic path for s IEF separation, and reconfigured for dividing separated bands. One microliter samples can be loaded and separated by s IEF into static bands in 10∼30 min. After a slipping operation, the static IEF bands can be divided into nanoliter droplets in microwells without mobilization, and the device can be opened for in situ MALDI ‐ TOF MS detection without loss of separation resolution. The performance of the device is characterized by separating and identifying intact proteins. The applicability in metabolic analysis is demonstrated by preliminary experiments on profiling small molecular metabolites in cerebrospinal fluid microdialysates from rat brain.