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Velocity gap mode of capillary electrophoresis developed for high‐resolution chiral separations
Author(s) -
Li Xue,
Li Youxin,
Zhao Lumeng,
Shen Jianguo,
Zhang Yong,
Bao James J.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400081
Subject(s) - analyte , capillary electrophoresis , enantiomer , resolution (logic) , chromatography , chemistry , capillary action , analytical chemistry (journal) , materials science , computer science , organic chemistry , artificial intelligence , composite material
A new CE method based on velocity gap (VG) theory has been developed for high‐resolution chiral separations. In VG, two consecutive electric fields are adopted to drive analytes passing through two capillaries, which are linked together through a joint. The joint is immersed inside another buffer vial which has conductivity communication with the buffer inside the capillary. By adjusting the field strengths onto the two capillaries, it is possible to observe different velocities of an analyte when it passes through those two capillaries and there would be a net velocity change (NVC) for the same analyte. Different analytes may have different NVC which may be specifically meaningful for enantioseparations because enantiomers are usually hard to resolve. By taking advantage of this NVC, it is possible to enhance the resolution of a chiral separation if a proper voltage program is applied. The feasibility of using NVC to enhance chiral separation was demonstrated in the separations of three pairs of enantiomers: terbutaline, chlorpheniramine, and promethazine. All separations started with partial separation in a conventional CE and were significantly improved under the same experimental conditions. The results indicated that VG has the potential to be used to improve the resolving power of CE in chiral separations.