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Chiral separation of 12 cathinone analogs by cyclodextrin‐assisted capillary electrophoresis with UV and mass spectrometry detection
Author(s) -
Merola Gustavo,
Fu Hanzhuo,
Tagliaro Franco,
Macchia Teodora,
McCord Bruce R.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400077
Subject(s) - chromatography , chemistry , cyclodextrin , mass spectrometry , capillary electrophoresis , analyte , detection limit , phosphate buffered saline , cathinone , analytical chemistry (journal) , amphetamine , neuroscience , dopamine , biology
In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin‐assisted CE with UV and TOF‐MS detection. Optimum separation was obtained on a 57.5 cm × 50 μm capillary using a buffer system consisting of 10 mM β‐cyclodextrin (β‐CD) in a 100 mM phosphate buffer for CE‐UV, and 0.6% v/v highly sulfated‐γ‐cyclodextrin (HS‐γ‐CD) in a 50 mM phosphate buffer for CE‐MS. In the CE‐MS experiment, a partial filling technique was employed to ensure that a minimum amount of cyclodextrin entered the mass spectrometer. All analytes were separated within 18 min in the CE‐UV separation and identified by TOF‐MS. Ten compounds were enantiomerically separated using β‐CD in the UV mode and an additional two more were enantiomerically separated using HS‐γ‐CD in the MS mode. Detection limits down to 1.0 ng/mL were obtained. The method was then applied to examine seized drugs.