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Investigator® A rgus X ‐12 study on the population of C zech R epublic: C omparison of linked and unlinked X ‐ STR s for kinship analysis
Author(s) -
Zidkova Anastassiya,
Capek Pavel,
Horinek Ales,
Coufalova Pavla
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400046
Subject(s) - argus , haplotype , population , biology , genetics , linkage (software) , genotype , demography , gene , computer science , sociology , programming language
DNA samples of 523 unrelated anonymized individuals (307 males and 216 females) born and living in the C zech R epublic were genotyped using Investigator® Argus X‐12 system in the following loci localized in four linkage groups: DXS 10148, DXS 10135, DXS 8378, DXS 7132, DXS 10079, DXS 10074, DXS 10103, HPRTB , DXS 10101, DXS 10146, DXS 10134, DXS 742. Haplotype frequencies were calculated for each LG (Linkage Group). The frequency of most common haplotype was 0.016, 0.036, 0.042, and 0.023 for LG 1, LG 2, LG 3, and LG 4, respectively. The combined power of discrimination was more than 0. both for female and male samples. The mean exclusion chance was 0. (trios) and 0. (duos). Informativity and suitability of Investigator® Argus X‐12 for kinship determination was assessed by computing in several female–female duos using LR (Likelihood Ratio) determination for autosomal STR (PowerPlex ESI ‐17), linked (Investigator® Argus X‐12 system), and unlinked ( X ‐ STR D ecaplex) X ‐ STR kits. Investigator® Argus X‐12 proved to be very useful for sibship determination, since its LR values were relatively similar to LR for autosomal STR kit. This work presents the first population data for Investigator® Argus X‐12 system in the C zech R epublic.