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Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin
Author(s) -
Jacroux Thomas,
Bottenus Danny,
Rieck Bennett,
Ivory Cornelius F.,
Dong Wenji
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400009
Subject(s) - cationic polymerization , chromatography , troponin i , isotachophoresis , chemistry , albumin , isoelectric focusing , electrophoresis , analytical chemistry (journal) , biochemistry , cardiology , enzyme , organic chemistry , electrolyte , medicine , electrode , myocardial infarction
Cationic ITP was used to separate and concentrate fluorescently tagged cardiac troponin I (cTnI) from two proteins with similar isoelectric properties in a PMMA straight‐channel microfluidic chip. In an initial set of experiments, cTnI was effectively separated from R‐Phycoerythrin using cationic ITP in a pH 8 buffer system. Then, a second set of experiments was conducted in which cTnI was separated from a serum contaminant, albumin. Each experiment took ∼10 min or less at low electric field strengths (34 V/cm) and demonstrated that cationic ITP could be used as an on‐chip removal technique to isolate cTnI from albumin. In addition to the experimental work, a 1D numerical simulation of our cationic ITP experiments has been included to qualitatively validate experimental observations.