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Application of temperature‐correlated mobility theory for optimizing the MEKC separation of the main lignans from Schisandra Chinensis Fructus and its prescription Yuye Decoction
Author(s) -
Liu Jingyi,
Petersen Nickolaj Jacob,
Lee KaiFai,
Hansen Steen Honoré,
Lao Lixing,
Sze Chowing,
Zhang Yanbo
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201400007
Subject(s) - decoction , schisandra chinensis , analyte , chemistry , chromatography , schisandra , traditional medicine , traditional chinese medicine , medicine , alternative medicine , pathology
The present work shows the application of the temperature‐correlated mobility theory for the optimization of the separation and peak alignment of the main lignans from water extracts of traditional Chinese medicine Schisandra Chinensis Fructus as well as its prescription Yuye Decoction (Jade Fluid Decoction; YYD). This is the first application of this theory for MEKC separations, and the data presentation allows a much easier peak tracking and thereby identification of the analytes. Most interestingly, the data obtained and presented in the mobility scale at 298 K, show that Schisantherin A, which is easily mistaken as one of the analytes using traditional time scale, was actually not detected in Schisandra Chinensis Fructus and Yuye Decoction (Jade Fluid Decoction) water extracts. This proves the value of the temperature‐correlated mobility scale for method optimization of complex samples. Thus, in the temperature‐correlated mobility scale, the optimization of the system conditions for the MEKC separations can easily be achieved by correcting for viscosity changes. Also, the influence of the operating temperature can be monitored in a more distinct way.