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Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture
Author(s) -
Huang Chao,
Santana Steven M.,
Liu He,
Bander Neil H.,
Hawkins Benjamin G.,
Kirby Brian J.
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201370191
Subject(s) - dielectrophoresis , microfluidics , prostate cancer , materials science , cancer cell , monoclonal antibody , electrode , characterization (materials science) , nanotechnology , cell , electrophoresis , adhesion , biophysics , cancer , chemistry , chromatography , biology , antibody , biochemistry , immunology , composite material , genetics
Electrophoresis 2013, 34 , 2970‐2979. DOI: 10.1002/elps.201300242 Characterization of adhesion of prostate cancer cells to immunocapture surfaces with and without dielectrophoresis (DEP) effects as a function of shear stress was performed in a Hele‐Shaw flow cell. Gold interdigitated electrodes were deposited on a glass surface that was functionalized with a prostate‐specific monoclonal antibody, J591; these electrodes applied an electric field gradient to attract prostate cancer cells to the immunocapture surface with positive DEP. This work demonstrates that DEP and immunocapture techniques can work synergistically to improve cancer cell capture performance, and it informs the design of future high‐purity rare cell capture systems to facilitate genetic and pharmacological evaluation of cancer.