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Coupling of fluorescence correlation spectroscopy with capillary and microchannel analytical systems and its applications
Author(s) -
Dong Chaoqing,
Ren Jicun
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300648
Subject(s) - microchannel , fluorescence correlation spectroscopy , capillary action , characterization (materials science) , nanotechnology , coupling (piping) , materials science , analytical chemistry (journal) , microfluidics , capillary electrophoresis , fluorescence spectroscopy , flow (mathematics) , nanoparticle , spectroscopy , fluorescence , chemistry , molecule , chromatography , mechanics , optics , physics , organic chemistry , quantum mechanics , metallurgy , composite material
In the past decade, fluorescence correlation spectroscopy (FCS) has become an ultrasensitive and noninvasive single‐molecule detection technique, which is widely applied in the physical, chemical, and life‐science research. The coupling of FCS with narrow channel flow systems including the ones based on capillary provide the important, convenient, and sensitive assay platforms for probing and understanding the behavior of single molecules or nanoparticles with improved temporal and spatial resolution and need for less sample volume among other advantages. This review focus on different approaches for FCS with capillary and microchannel analytical systems and its applications in confined diffusion study, flow profiles, and imaging of narrow channel, multicomponent analyses such as protein, DNA analysis, and characterization on nanoparticles.