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Polymer monolithic capillary column fabricated by using monodisperse iron oxide nanocrystal template to enhance the electrochromatographic separation of small molecules
Author(s) -
Lin Zian,
Wang Juan,
Yin Xiaofei,
Tan Xiaoqing,
Yu Ruifang,
Zheng Jiangnan,
Zhang Lan,
Chen Guonan
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300647
Subject(s) - glycidyl methacrylate , monolith , materials science , nanocrystal , polymer , dispersity , methacrylate , chemical engineering , mesoporous material , polymerization , monomer , nanotechnology , polymer chemistry , chemistry , organic chemistry , composite material , catalysis , engineering
Monodisperse iron oxide nanocrystals and organic solvents were utilized as coporogens in monolithic poly(glycidyl methacrylate‐ co ‐ethylene glycol dimethacrylate) capillary columns to afford stationary phases with enhanced electrochromatographic performance of small molecules. While the conventional monoliths using organic solvents only as a porogen exhibited poor resolution (Rs) <1.0 and low efficiency of 40 000–60 000 plates/m, addition of a small amount of nanocrystals to the polymerization mixture provided increased resolution (Rs > 3.0) and high efficiency ranged from 60 000 to 100 000 plates/m at the same linear velocity of 0.856 mm/s. It was considered that the mesopores introduced by the nanocrystals played an important role in the improvement of the monolith performance. This new strategy expanded the application range of the hydrophobic monoliths in the separation of polar alkaloids and narcotics. The successful applications demonstrated that the glycidyl methacrylate based monoliths prepared by using nanocrystal template are a good alternative for enhanced separation efficiency of small molecules.