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Improving sensitivity in microchip electrophoresis coupled to ESI‐MS/MS on the example of a cardiac drug mixture
Author(s) -
Schwarzkopf Fabian,
Scholl Tobias,
Ohla Stefan,
Belder Detlev
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300615
Subject(s) - chromatography , chemistry , sensitivity (control systems) , drug , analytical chemistry (journal) , pharmacology , medicine , electronic engineering , engineering
A comprehensive study for a sensitivity optimization in MCE with mass spectrometric detection is presented. As a text mixture, we chose a mixture of the cardiac drugs propranolol, bisoprolol, lidocaine, procaine and studied the effect of different chip layouts and experimental parameters with the aim of achieving both high sensitivity in MS detection and adequate chip electrophoretic separation. An important aspect was a comparison of microfluidic layouts containing various sheath‐flow channels with that avoiding sheath‐flow junctions on‐chip. We utilized glass chips with monolithically integrated nanospray emitter tips coupled dead volume‐free to an IT mass spectrometer running in fragmentation mode (MS n ). With this setup, detection limits down to 0.6 ng/mL for the model compound propranolol were achieved.