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Genetic polymorphisms of 20 short tandem repeat loci from the Han population in Henan, China
Author(s) -
Wang HongDan,
Wu Dong,
Feng ZhanQi,
Jing ZhiAn,
Li Tao,
Guo QianNan,
Zhang XianPing,
Hou QiaoFang,
Guo LiangJie,
Kang Bing,
Zhang Hui,
Zhu BoFeng,
Liao ShiXiu
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300597
Subject(s) - genetics , biology , microsatellite , genotype , locus (genetics) , allele frequency , allele , population , genotype frequency , gene , demography , sociology
The aim of this study was to investigate the genetic polymorphism of 20 short tandem repeat (STR) loci including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX, and vWA in Han population of Henan, China and to assess its value in forensic science. Genomic DNA was extracted from 274 blood samples of unrelated healthy individuals in the Henan Han population. Alleles were amplified with PowerPlex® 21 system kit and PCR products were detected with ABI3130 genetic analyzer (Applied Biosystems) and the data were analyzed with modified PowerStats v1.2. A total of 229 alleles were observed in this Han population and the allelic frequencies ranged from 0.0020 to 0.5090 in the present study. Observed genotype distributions for each locus do not show deviations from Hardy–Weinberg equilibrium expectations ( p < 0.05). The combined power of discrimination, combined power of exclusion, and combined matching probability of this 20 STR loci were 0., 0.4603, and 4.0433 × 10 −24 , respectively. The 20 STR loci are highly polymorphic in the Han population of Henan, China and they may be of great value in forensic science and human population genetics.