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Combination of dynamic pH junction with capillary electrophoresis‐mass spectrometry for the determination of systemins in plant samples
Author(s) -
Bai Yu,
Chang Cuilan,
Du Fuyou,
Tan Zhijing,
Bai Yu,
Liu Huwei
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300434
Subject(s) - capillary electrophoresis , mass spectrometry , chromatography , capillary electrophoresis–mass spectrometry , chemistry , analytical chemistry (journal) , electrophoresis , capillary action , materials science , electrospray ionization , composite material
Systemin is an important group of plant peptide hormones participating in the regulation of plant defensive responses. An improved method, based on dynamic pH junction and capillary electrophoresis‐quadrupole time‐of‐flight mass spectrometry, was developed for online enrichment and sensitive determination of trace systemins in plants. After optimization, the online enrichment factors for six target systemins ranged from 90‐ to 127‐fold. The detection limits reached lower than 0.5 nM, which were comparable with the sensitivity of LC‐MS method. Satisfactory quantitative results were obtained in terms of linearity ( R 2 ≥ 0.993), dynamic range (3–120 ng/mL), and reproducibility (≤6.7%). For the analysis of real plant samples, a rapid sample preparation method was developed, using two steps of SPE purification with different retention and separation mechanisms. Finally, this method realized the successful detection of tomato systemin and tobacco hydroxyproline‐rich systemin I from plant leaves with shorter analysis time.