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ITP of lanthanides in microfluidic PMMA chip
Author(s) -
Cong Yongzheng,
Bottenus Danny,
Liu Bingwen,
Clark Sue B.,
Ivory Cornelius F.
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300382
Subject(s) - lanthanide , electrolyte , microchannel , acetic acid , multiphysics , materials science , chemistry , nuclear chemistry , chromatography , ion , electrode , nanotechnology , organic chemistry , physics , finite element method , thermodynamics
An ITP separation of eight lanthanides on a serpentine PMMA microchip with a tee junction and a 230‐mm‐long serpentine channel is described. The cover of the PMMA chip is 175 μm thick so that a C 4 D in microchip mode can be used to detect the lanthanides as they migrate through the microchannel. Acetate and α‐hydroxyisobutyric acid are used as complexing agents to increase the electrophoretic mobility difference between the lanthanides. Eight lanthanides are concentrated within ∼ 6 min by ITP in the microchip using 10 mM ammonium acetate at pH 4.5 as the leading electrolyte and 10 mM acetic acid at ∼ pH 3.0 as the terminating electrolyte. In addition, a 2D numerical simulation of the lanthanides undergoing ITP in the microchip is compared with experimental results using COMSOL Multiphysics v4.3a.