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N ‐methyl‐2‐pyrrolidonium methyl sulfonate acidic ionic liquid as a new dynamic coating for separation of basic proteins by capillary electrophoresis
Author(s) -
Guo XiaoFeng,
Chen HuiYing,
Zhou XiaoHai,
Wang Hong,
Zhang HuaShan
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300369
Subject(s) - coating , capillary action , chemistry , adsorption , lysozyme , electrolyte , sulfonate , chromatography , hydrogen bond , electrophoresis , ionic liquid , chemical engineering , materials science , molecule , organic chemistry , composite material , sodium , electrode , biochemistry , engineering , catalysis
A simple and economical CE method has been developed for the analysis of four model basic proteins by employing N ‐methyl‐2‐pyrrolidonium methyl sulfonate ionic liquid (IL) as the dynamic coating material based on the interaction of both between electrostatic attraction and hydrogen bond, and between the organic cations of IL and the inner surface of bare fused‐silica capillary. The N ‐methyl‐2‐pyrrolidonium‐based IL modified capillary not only generated a stable suppressed electroosmotic flow, but also effectively eliminated the wall adsorption of proteins. Several important parameters such as the IL concentration, pH values, and concentrations of the background electrolyte were optimized to improve the separation of basic proteins. Consequently, under the optimum separation conditions, a satisfied separation of basic proteins including lysozyme, cytochrome c , ribonuclease A, and α‐chymotrypsinogen A with theoretical plates ranging from 2.09 × 10 5 to 4.48 × 10 5 plates/m had been accomplished within 15 min. The proposed method first illustrated the effect of hydrogen bond between coating material and inner capillary surface on the coating, which should be a new strategy to design and select more effective coating materials to form more stable coatings in CE.