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CD 133 and CD 133‐regulated nucleophosmin linked to 5‐fluorouracil susceptibility in human colon cancer cell line SW 620
Author(s) -
Kim KyungHee,
Yoo Byong Chul,
Kim Won Ki,
Hong Jun Pyu,
Kim Kun,
Song Eun Young,
Lee Jae Yong,
Cho Jae Youl,
Ku JaLok
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300364
Subject(s) - nucleophosmin , colorectal cancer , proteome , cell culture , chemotherapy , cancer research , cancer , microbiology and biotechnology , biology , chemistry , biochemistry , genetics , myeloid leukemia
Cancer stem cells ( CSC s) are known to be resistant to conventional chemotherapy and radiotherapy. Specific CSC targeting and eradication is therefore a therapeutically important challenge. CD 133 is a colorectal CSC marker with unknown function(s). Assessing proteomic changes induced by CD 133 may provide clues not only to new CD 133 functions but also to the chemotherapy and radiation susceptibility of colon cancer cells. To identify the proteins affected by CD 133, CD 133‐positive ( CD 133+), and CD 133‐negative ( CD 133–) human colon cancer cells were obtained by cell sorting. Whole proteomes were profiled from SW 620/ CD 133+ and SW 620/ CD 133– cells and analyzed by 2 D ‐based proteome analysis. Nucleophosmin ( NPM 1) was identified as a protein regulated by CD 133. CD 133 protein level was not affected by NPM 1, and an interaction between the two proteins was not observed. CD 133 and NPM 1 protein levels were positively correlated in 11 human colon cancer cell lines. The CD 133+ subpopulation percentage or its value normalized against CD 133 protein level was only linked to intrinsic susceptibility of human colon cancer cells to 5‐fluorouracil (5‐ FU ). However, either suppression of CD 133 or NPM 1 significantly increased 5‐ FU susceptibility of SW 620. The present study suggests that CD 133‐regulated NPM 1 protein level may provide a clue to novel CD 133 function(s) linked to human colon cancer cell susceptibility to chemotherapy.