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Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using S tains‐ A ll
Author(s) -
Cong WeiTao,
Ye WeiJian,
Chen Mao,
Zhao Ting,
Zhu ZhongXin,
Niu Chao,
Ruan Dandan,
Ni MaoWei,
Zhou Xuan,
Jin LiTai
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300328
Subject(s) - stain , staining , phosvitin , chemistry , chromatography , negative stain , microbiology and biotechnology , biology , biochemistry , phosphorylation , protein kinase a , electron microscope , physics , optics , genetics
An improved S tains‐ A ll ( ISA ) staining method for phosphoproteins in SDS ‐ PAGE was described. Down to 0.5–1 ng phosphoproteins (α‐casein, β‐casein, or phosvitin) can be successfully selectively detected by ISA stain, which is approximately 120‐fold higher than that of original S tains‐ A ll stain, but is similar to that of commonly used P ro‐ Q D iamond stain. Furthermore, unlike the original S tains‐ A ll protocol that was time consuming and light unstable, ISA stain could be completed within 60 min without resorting to protect the gels from light during the whole staining procedure. According to the results, it is concluded that ISA stain is a rapid, sensitive, specific, and economic staining method for a broad application to the research of phosphoproteins.