Premium
Growth inhibition and differences in protein profiles in azadirachtin‐treated Drosophila melanogaster larvae
Author(s) -
Wang Hao,
Lai Duo,
Yuan Mei,
Xu Hanhong
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300318
Subject(s) - azadirachtin , drosophila melanogaster , biopesticide , biology , larva , melanogaster , insect , heat shock protein , growth inhibition , mode of action , pest analysis , biochemistry , microbiology and biotechnology , botany , gene , cell growth , pesticide , ecology
Azadirachtin A is a very effective biopesticide widely used in insect pest control. It has strong antifeeding and growth inhibitory activity against most insects, however, its mode of action is still unclear. Proteomic experiments using 2DE indicate significant effects of Azadirachtin A on the amount of proteins related to growth inhibition in Drosophila melanogaster larvae. Twenty‐one spots with different intensity in azadirachtin‐treated larvae were identified. These proteins are involved in cytoskeletal organization, transcription and translation, hormonal regulation, and energy metabolism. Protein network analysis reveals heat shock protein 23 to be a potential target of azadirachtin. These results provide new insights into understanding the mechanism of growth inhibition in insects in response to azadirachtin.