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Evaluation of electroosmotic markers in aqueous and nonaqueous capillary electrophoresis
Author(s) -
Hellqvist Alexander,
Hedeland Ylva,
Pettersson Curt
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300305
Subject(s) - capillary electrophoresis , chromatography , aqueous solution , chemistry , capillary action , electrophoresis , analytical chemistry (journal) , materials science , organic chemistry , composite material
The most common method to determine the EOF in CE is to measure the migration time for a neutral marker. In this study, 12 compounds (three novel and some previously used) were investigated as EOF markers in aqueous and nonaqueous BGE s. In the aqueous buffer systems (ammonium acetate, sodium phosphate, and sodium borate) the evaluation included a wide pH range (2–12). Two BGE s contained chiral selectors (sulphated‐β‐ CD , (−)‐diketogulonic acid) and one that contained a micellar agent ( SDS ) were included in the study. The majority of the evaluated compounds were found to migrate with the EOF in the water‐based BGE s and are thus useful as EOF markers. However, in the SDS ‐based BGE only four of the compounds (acetone, acrylamide, DMSO , and ethanol) were found to be applicable. In the nonaqueous BGE s 11 markers (acetone, acetophenone, acrylamide, anthracene, benzene, 4‐(4‐methoxybenzylamino)‐7‐nitro‐2,1,3‐benzoxadiazole, benzyl alcohol, 2,5‐diphenyloxazole, ethanol, flavone, and mesityl oxide) seemed to be functional as EOF markers. Even though several of the evaluated compounds can be used as EOF markers in the investigated BGE s, the authors would recommend the use of acrylamide as a general marker for UV detection. Furthermore, the four fluorescent markers (of which three were novel) gave RSD values equal to the other markers and can be used for the determination of the EOF in CE or microchip CE with fluorescence detection.