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Electrolyte system strategies for anionic isotachophoresis with electrospray‐ionization mass‐spectrometric detection. 1. Regular isotachophoresis and free‐acid isotachophoresis
Author(s) -
Malá Zdena,
Gebauer Petr,
Boček Petr
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300292
Subject(s) - isotachophoresis , chemistry , electrospray ionization , electrolyte , chromatography , formic acid , stacking , electrospray , mass spectrometry , analytical chemistry (journal) , organic chemistry , electrode
The subject of this work is the definition of a simple model based on general ITP theory that allows describing and predicting the behavior of ITP systems compatible with ESI ‐ MS detection. The model is exemplified by anionic ITP of weak acids that represent an interesting potential application field of ITP ‐ ESI ‐ MS . Suitable ESI ‐compatible electrolyte systems of very simple composition are proposed including a special free‐acid ITP arrangement. The properties of these systems are discussed using illustrative diagrams of their stacking windows. The use of anionic ITP ‐ ESI ‐ MS in negative‐ion ESI mode is reported for the first time and its suitability for sensitive trace analysis is demonstrated. The presented ITP ‐ ESI ‐ MS application example comprises a free‐acid ITP system formed of formic and propionic acids and direct injection analysis of ibuprofen and diclofenac in waters with quantitation limits of the order 10 −10 M.