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Differentiation of strains from the B acillus cereus group by RFLP ‐ PFGE genomic fingerprinting
Author(s) -
Otlewska Anna,
OltuszakWalczak Elzbieta,
Walczak Piotr
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300246
Subject(s) - bacillus cereus , cereus , biology , bacillus thuringiensis , pulsed field gel electrophoresis , microbiology and biotechnology , dna profiling , bacillus anthracis , restriction fragment length polymorphism , bacillaceae , bacillus (shape) , genetics , genotype , bacteria , dna , gene , bacillus subtilis
Bacillus mycoides , B acillus pseudomycoides , B acillus weihenstephanensis , B acillus anthracis , B acillus thuringiensis , and B acillus cereus belong to the B. cereus group. The last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. The macrorestriction analysis of the genomic DNA with the N ot I enzyme was used to generate polymorphism of restriction profiles for 39 food‐borne isolates ( B . cereus , B . mycoides ) and seven reference strains ( B. mycoides , B . thuringiensis , B . weihenstephanensis , and B . cereus ). The PFGE method was applied to differentiate the examined strains of the B . cereus group. On the basis of the unweighted pair group method with the arithmetic mean method and D ice coefficient, the strains were divided into five clusters (types A – E ), and the most numerous group was group A (25 strains). A total of 21 distinct pulsotypes were observed. The RFLP ‐ PFGE analysis was successfully used for the differentiation and characterization of B . cereus and B . mycoides strains isolated from different food products.