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Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
Author(s) -
Svobodova Zuzana,
Kucerova Jana,
Autebert Julien,
Horak Daniel,
Bruckova Lenka,
Viovy JeanLouis,
Bilkova Zuzana
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300196
Subject(s) - chip , lab on a chip , chemistry , circulating tumor cell , computer science , nanotechnology , materials science , cancer , microfluidics , medicine , telecommunications , metastasis
In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide‐PEG‐hydrazide, and subsequently anti‐epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti‐EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line.