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Particles small angle forward‐scattered light measurement based on photovoltaic cell microflow cytometer
Author(s) -
Chen HanTaw,
Fu LungMing,
Huang HsingHui,
Shu WeiEn,
Wang YaoNan
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300189
Subject(s) - optics , scattering , laser , light scattering , forward scatter , signal (programming language) , materials science , particle (ecology) , microfluidics , laser power scaling , range (aeronautics) , multiangle light scattering , optoelectronics , physics , nanotechnology , oceanography , composite material , computer science , programming language , geology
A method is proposed for detecting microparticles in a microflow cytometer by means of small angle forward‐scattered light measurements. The proposed cytometer comprises a commercial photovoltaic cell, an adjustable power laser module, and a PDMS microfluidic chip. The detection performance of the proposed device is evaluated using particles with dimensions of 5, 8, 10, and 15 μm, respectively, given forward‐light scattering angles of 5 and 8° and laser powers ranging from 15–25 mW. It is shown that for a constant laser power and particle size, the S / N of the detected light signal increases with a reducing forward‐scattering angle. Moreover, for a constant forward‐scattering angle and particle size, the S / N increases with an increasing laser power. The intensity of the forward‐scattered light signal is found to vary linearly with the particle size and has a correlation coefficient of R 2 = 0.967, 0.967, and 0.963 given laser powers of 15, 20, and 25 mW, respectively, and a forward‐scattering angle of 5°. Moreover, the CV of the forward‐scattered light intensity is found to lie within the range of 20–30% for both forward‐scattering angles. Overall, the present results suggest that the proposed device has significant potential for detection applications in the medical, environmental monitoring, and biological science fields