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Oral cancer secretome: Identification of cancer‐associated proteins
Author(s) -
Chang HongYun,
Hor SeenYii,
Lim KuePeng,
Zain Rosnah Binti,
Cheong SokChing,
Rahman Mariati Abdul,
Karsani Saiful Anuar
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300126
Subject(s) - carcinogenesis , cancer , biology , cancer cell , in silico , telomerase reverse transcriptase , cell culture , telomerase , cell , proteomics , cell growth , cancer research , microbiology and biotechnology , gene , genetics
This study aims to identify cancer‐associated proteins in the secretome of oral cancer cell lines. We have successfully established four primary cell cultures of normal cells with a limited lifespan without human telomerase reverse transcriptase (h TERT ) immortalization. The secretome of these primary cell cultures were compared with that of oral cancer cell lines using 2 DE . Thirty five protein spots were found to have changed in abundance. Unambiguous identification of these proteins was achieved by MALDI TOF / TOF . In silico analysis predicted that 24 of these proteins were secreted via classical or nonclassical mechanisms. The m RNA expression of six genes was found to correlate with the corresponding protein abundance. Ingenuity Pathway Analysis ( IPA ) core analysis revealed that the identified proteins were relevant in, and related to, cancer development with likely involvements in tumor growth, metastasis, hyperproliferation, tumorigenesis, neoplasia, hyperplasia, and cell transformation. In conclusion, we have demonstrated that a comparative study of the secretome of cancer versus normal cell lines can be used to identify cancer‐associated proteins.