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A new approach to the studies of submicron particle suspensions based on the effect of pressure in capillary zone electrophoresis
Author(s) -
Vanifatova Nataliya,
Rudnev Alexander,
Spivakov Boris
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300118
Subject(s) - capillary electrophoresis , particle (ecology) , electrophoresis , capillary action , chemistry , particle size , analytical chemistry (journal) , thermal diffusivity , chromatography , suspension (topology) , acceleration , electrokinetic phenomena , mechanics , materials science , thermodynamics , composite material , classical mechanics , oceanography , physics , mathematics , homotopy , pure mathematics , geology
A new approach based on the effect of pressure in CZE is suggested for acceleration of particle migration in electrophoretic runs resulting in reduction of the analysis time. It provides conditions for studying fast processes in suspensions. The effect of pressure on the migration of silica spheres with average diameters of 100, 150, and 390 nm was studied by CZE at an applied voltage of 25 kV. The particle hydrodynamic behavior was also investigated under the same capillary dimensions and BGE composition. The total particle mobility (excluding the average flow rate) was found to increase with increasing the pressure applied and particle size. The particle migration mechanism explaining the effect of pressure on particle velocity was shown to be almost the same as in wide‐bore hydrodynamic chromatography. It is based on changing radial distribution of particle concentration along the capillary cross section depending on particle diffusivity. On the basis of this mechanism appearance of a zone of negatively charged particles in electropherograms ahead of the marker peak can be explained.