Premium
Application of capillary electrophoretic chips in protein profiling of plant extracts for identification of genetic modifications of maize
Author(s) -
Poboży Ewa,
Filaber Monika,
Koc Anna,
GarciaReyes Juan F.
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300103
Subject(s) - capillary electrophoresis , profiling (computer programming) , electrophoresis , chromatography , identification (biology) , chemistry , biology , computational biology , botany , computer science , operating system
In this study, the chip gel electrophoresis with LIF detection was applied in protein profiling of fractionated and total extracts of maize standards. The sensitivity of such determinations can be enhanced by lyophilization of extracts or employing filtering and preconcentration with cutoff filters. Combinatorial peptide ligand library applied for sample processing prior to the electrophoretic analysis was, especially, an effective pretreatment step in the determination of low‐abundance proteins. Several repeatable differences were observed for protein profiles between maize standards not containing the genetically modified organisms (GMOs) and those containing GMO, which can be potentially employed for identification of GMO in maize samples and foods of maize origin.