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Determination of nitrogen mustard degradation products in water samples using a portable capillary electrophoresis instrument
Author(s) -
Sáiz Jorge,
Mai Thanh Duc,
Hauser Peter C.,
GarcíaRuiz Carmen
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300054
Subject(s) - repeatability , capillary electrophoresis , analyte , chromatography , capillary action , coating , chemistry , degradation (telecommunications) , matrix (chemical analysis) , reproducibility , analytical chemistry (journal) , materials science , composite material , computer science , telecommunications , organic chemistry
In this work, a new purpose‐made portable CE instrument with a contactless conductivity detector was used for the determination of degradation products of nitrogen mustards in different water samples. The capillary was coated with poly(1‐vinylpyrrolidone‐co‐2‐dimethylaminoethyl methacrylate) to avoid analyte‐wall interactions. The coating procedure was studied to obtain the best repeatability of the migration time of the analytes. Four different coating procedures were compared; flushing the capillary with the copolymer at 100 psi for 2 min at 60°C provided the best RSD values (<4%). The analytical method was also optimized. The use of 20 mM of MES adjusted to pH 6.0 with His as running buffer allowed a good baseline separation of the three analytes in different water samples without matrix interferences. The method permitted the detection of the three degradation products down to 5 μM.