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Comparative proteomic analysis of different T oxoplasma gondii genotypes by two‐dimensional fluorescence difference gel electrophoresis combined with mass spectrometry
Author(s) -
Zhou DongHui,
Zhao FuRong,
Nisbet Alasdair J.,
Xu MinJun,
Song HuiQun,
Lin RuiQing,
Huang SiYang,
Zhu XingQuan
Publication year - 2014
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300044
Subject(s) - toxoplasma gondii , biology , heat shock protein , kegg , difference gel electrophoresis , microbiology and biotechnology , apicomplexa , genotype , strain (injury) , metabolic pathway , gene , proteomics , biochemistry , genetics , gene expression , plasmodium falciparum , transcriptome , antibody , malaria , anatomy , immunology
Toxoplasma gondii is a protozoan parasite infecting almost all warm‐blooded animals and humans. There are three infective stages of T. gondii : the tachyzoites, the bradyzoites, and the oocysts. The tachyzoite is a rapidly multiplying stage and the main pathogenic factor. In N orth A merica and E urope, T. gondii is consisted of four major clonal lineages (namely Types I, II, III, and Type 12). In this study, we explored the proteomic profiles of different genotypes (Type I‐ RH strain, Type II‐ PRU strain, Type II‐ T g QHO strain, and T oxo DB 9‐TgC7 strain) of T. gondii tachyzoites by using 2D DIGE combined with MALDI ‐ TOF MS . Totally, 110 differentially abundant protein spots were selected. Of these, 98 spots corresponding to 56 proteins from T. gondii were successfully identified. These included surface antigen ( SAG 1), heat shock protein 70 ( H sp 70), disulfide isomerase, coronin, heat shock protein 60 ( H sp 60), pyruvate kinase, receptor for activated C kinase 1, and peroxiredoxin. Gene ontology enrichment analysis revealed that most of the differentially abundant proteins were involved in biological regulation, metabolic process, response to stress, binding, antioxidant activity, and transporter activity. According to the KEGG metabolic pathway maps of T. gondii , some identified proteins were involved in the glycolytic/gluconeogenesis pathway. The present study identified differentially abundant proteins among different genotypes of T. gondii and these findings have implications for the better understanding of the phenotypic differences among the examined T. gondii genotypes, which in turn may contribute to the better control of toxoplasmosis.