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Preparation of chitosan‐graft‐(β‐cyclodextrin) based sol‐gel stationary phase for open‐tubular capillary electrochromatography
Author(s) -
Lü Haixia,
Li Qingyin,
Yu Xiaowei,
Yi Jiaojiao,
Xie Zenghong
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300035
Subject(s) - capillary electrochromatography , chitosan , chromatography , capillary action , cyclodextrin , chemistry , electrochromatography , stationary phase , thiourea , sol gel , silica gel , phase (matter) , selectivity , materials science , nanotechnology , catalysis , organic chemistry , composite material
A novel open‐tubular CEC column coated with chitosan‐graft‐(β‐CD) ( CDCS ) was prepared using sol‐gel technique. In the sol‐gel approach, owing to the 3 D network of sol‐gel and the strong chemical bond between the stationary phase and the surface of capillary columns, good chromatographic characteristics and unique selectivity in separating isomers were shown. The column efficiencies of 55 000∼163 000 plates/m for the isomeric xanthopterin and phenoxy acid herbicides using the sol‐gel‐derived CDCS columns were achieved. Good stabilities were demonstrated that the RSD values for the retention time of thiourea and isoxanthopterin were 1.3 and 1.4% (run to run, n = 5), 1.6 and 2.0% (day to day, n = 3), 2.9 and 3.1% (column to column, n = 3), respectively. The sol‐gel‐coated CDCS columns have shown improved separations of isomeric xanthopterin in comparison with CDCS ‐bonded capillary column.